Proteomics-guided characterization of Hericium erinaceus protein-capped silver nanoparticles for cytotoxicity against cancer cells and improved hemocompatibility

Information from the abstract

Biological synthesis offers an alternative approach for producing silver nanoparticles (AgNPs) using naturally derived biomolecules. In this study, a protein-rich extract of Hericium erinaceus (PHEx) was used for the biosynthesis of protein-capped AgNPs, followed by physicochemical, proteomic, cytotoxicity, and hemocompatibility analyses. The AgNPs exhibited a surface plasmon resonance band at approximately 410 nm, a hydrodynamic diameter of 70-90 nm, and a moderately negative surface charge. FTIR and proteomic analyses indicated the association of extract-derived biomolecules, particularly proteins, with the nanoparticle surface, supporting the formation of a protein-associated capping layer. Comparative LC-MS/MS identified 2,374 proteins, with 1,203 shared between PHEx and the AgNPs-associated fraction, further supporting the involvement of PHEx-derived proteins in AgNPs surface capping and stabilization. The AgNPs showed concentration-dependent cytotoxicity against SW480, NCI-N87, and HepG2 cells, with IC 50 values of 30.63, 23.21, and 2.01 µg/mL, respectively, compared with 223.19 µg/mL in CCD 841 CoN cells. AgNPs treatment was associated with increased total oxidative status and changes in Bax and Bcl-2 mRNA expression. Hemolysis remained below 1% up to 250 µg/mL. Collectively, PHEx-derived protein-capped AgNPs showed defined physicochemical characteristics, in vitro cytotoxic activity, and low hemolytic activity under the conditions examined.

Why this record is monitored

This record has an Impact Signal of 74/100 based on recency, source, collaboration, and bibliographic signals. It prioritizes monitoring and is not a judgment of research quality.

Related topics: Fungal Biology and Applications · Silymarin and Mushroom Poisoning · Polysaccharides and Plant Cell Walls